Shortly thereafter, the freeze-dry process was applied to penicillin and bone, and lyophilization became recognized as an important technique for preservation of biologicals.
Rotary evaporation for high boiling hydrogen bond-forming solvents such as water is often a last recourse, as other evaporation methods or freeze-drying (lyophilization) are available.
At this time, a new process called lyophilization was being introduced and was already highly acclaimed for preserving food animals with optimal retention of their nutrients and vitamins.